德国亥姆霍兹环境研究中心Beate Escher 教授学术报告

报告题目：

Dynamics of urban and agricultural organic micropollutants and their influence on the water quality of a small creek

专家介绍：

Beate Escher 教授是德国亥姆霍兹环境研究中心（UFZ）细胞毒理学系主任，德国图宾根大学环境毒理学教授，同时还是瑞士联邦工学院、澳大利亚Queensland大学和Griffith大学客座教授。她是德国自然科学和人文科学理事会委员，现任国际顶级期刊 Environmental Science & Technology 副主编。

报告摘要：

Rivers integrate water of their entire catchment and pose an important environmental compartment in which transport and transformation processes of anthropogenic chemicals occur. To trace changes in the water quality and to characterize potential input sources of organic micropollutants an integrated analytical and bioanalytical approach was applied. A small creek, the Ammer river, Tübingen (Germany), was sampled in its entirety using grab sampling and in a more detailed follow up study at two sampling sites with large volume-solid phase extraction (LV-SPE) devices. Both LV-SPE devices integrated a total period of 24 hours divided in four sampling intervals. The water extracts were measured by four in-vitro bioassays covering the environmentally relevant mode of actions of activation of the aryl hydrocarbon receptor (AhR), estrogenicity (ER), oxidative stress response (AREc32) and mitochondrial toxicity measured by the cellular oxygen consumption rate (OCR) assay using the Agilent Seahorse XF^e96 Analyzer. Furthermore, all samples were screened for close to one hundred target analytes by LC-MS/MS. The water extracts showed temporal and spatial variability in the assays AhR and AREc32 with most samples being active. The OCR assay showed initial uncoupling activity at the upstream sampling site, just below a wastewater treatment plant, which transitioned to marginal uncoupling at the same sampling site at later sampling periods and strong electron transport chain inhibition at the downstream site. The uncoupling effect coincided with estrogenic activity, which is a marker of the water being dominated by wastewater treatment plant effluent. In all other samples and the tributaries the mitochondrial toxicity was dominated by the inhibition of the electron transport chain. This study shows the dynamics of organic micropollutants in one stretch of a small creek and demonstrates that the impact of anthropogenic chemicals on the water quality maybehighly variable. This work was supported by the Collaborative Research Centre 1253 CAMPOS (Project P1: Rivers), funded by the German Research Foundation (DFG, Grant Agreement SFB 1253/1 20147).